No. A transfer is a financial event, not work permission. The actual E-2 conditions and appropriate visa and admission process remain necessary.
Coordinate commitment with the real immigration steps
Canadians generally need an E visa. Describe the intended activity and actual status rather than treating the payment date as an authorized work start.
Work backwards from the steps you control: covenant wording settled, funds traced and transferred, closing completed or firmly committed, business plan and staffing set, packet reviewed, appointment attended. Signing the lease, ordering equipment, arranging utilities and licensing, engaging a local manager and hiring staff all constitute irrevocable commitment and make the application stronger rather than weaker. An escrow whose only outstanding condition is issuance of the visa is accepted in official guidance as a solid, irrevocable commitment, while one the buyer can unwind for reasons of their own has preserved the funds rather than committed them. Some qualifying arrangements involve an investment-in-process or escrow structure, so the proposed terms should be reviewed before deciding on the sequence rather than assuming every purchase must be fully closed first.